Peptide & Scaffold Leads Discovery (PSLD) Platform
PSLD cDNA display is an in-vitro novel screening technology for biological targets by using a puromycin-linker DNA containing a ‘ligation site’, a ‘biotin site’ ‘reverse transcription primer site’ and a ‘restriction enzyme site’.
The puromycin–linker DNA characteristics features facilitate the efficient synthesis of stable mRNA/cDNA–protein fusion molecules.
PSLD display enables in-vitro selection of various kinds of peptide and protein libraries of more than 1~10 trillion different sequences which is higher than other in vitro display techniques.
PSLD PUROMYCIN LINKER
➀ strong covalent bonding via the puromycin
➁ biotin moiety for the solid phase immobilization of the mRNA-linker, allowing post-translational chemical or enzymatic crosslinking, various modifications and reverse transcription reaction with non-natural amino acids
➂ the linker harboring a primer for reverse transcription, enabling rapid synthesis of cDNA